Proteintech Learning Portal/Introduction to R for Life Scientists

Introduction to R for Life Scientists

This beginner friendly R coding course will teach you how to perform accurate and robust immunoassay data analysis. 

This course will help you understand the benefits of using R for complex data analysis, how to create reproducible data workflows, and showcase step-by-step analysis examples using real-world biological data.

Meet your tutor

Dr Toryn Poolman is a lecturer and biostatistician working at University College London. He has extensive experience working with complex biological systems and specialises in applying omics techniques, including RNAseq, phospho-proteomics and microbiome analysis.

Contents

Welcome

Introduction

Chapter 1: An introduction to R and Google Colab

This chapter will focus on the basics of the programming language R and how it can be used with Google Colab, a free cloud-based data processing suite.

In this chapter, we will run through real world data examples. All of the files are available below for you to try yourselves. You can download the raw datafiles or use our Github link.

https://github.com/ProteintechLab/Statistics

Introduction to R and Google Colab
How to set-up R in Google Colab
Importing Data
Manipulating Data
Summarizing Data

Chapter 2: Data Visualization in R

In this chapter, we will learn how to create plots using the ggplot2 package which is include in the pre-installed tidyverse package.

GGPLOT2 package in R
How to create graphs in R using GGPLOT2
Cowplot Package
Good Practice for Biological Data Visualization

Chapter 3: Example Data Analysis

In this chapter, we will use example datasets from different immunoassay applications to show you how to analyse using Google Colab.

The datasets and the jupyter notebooks used are available to you on Github (https://github.com/ProteintechLab/Statistics/tree/main) to help follow along with the analysis.

Western Blot
ELISA
Intensity Values (e.g., bead array)
qPCR
T Test
ANOVA

Summary

Congratulations on completing the Proteintech Learning Portal Introduction to R for Life Scientists course.

Let's recap what we have learned.

R Cheat Sheet
Learning Checklist

Logo is adapted from “R logo” by The R Foundation. Used and adapted under CC-BY-SA 4.0 by Proteintech.